Tumor cells and inoculation
DLA cells were obtained from Amala Cancer Research Centre ( Trissur, Kerala, India). The cells were administered to Swiss albino mice, by i.p. transplantation. Tumor cells aspirated from the peritoneal cavity of mice were washed with saline and were given i.p. to develop as acites tumor to all the animals except the normal group.
Materials, diet used, source of fine chemicals and experimental setup are as in 2.2.1, 18.104.22.168, 22.214.171.124, 126.96.36.199 and 188.8.131.52 repectively. Collection of mice tissue and preparation of tissue homogenate are as in section 7.2.1 and 7.2.2 respectively.
Anti-oxidant activity on lymphoma-induced mice
Animals were grouped into three groups consisting of nine mice each.
Group I - Normal group which received only normal lab diet (Gold Mohur mouse
Group II - Control group which received intraperitoneal injections of 1x105
Dalton’s lymphoma cells (DLA) and not treated.
Group III - Experimental group which received intraperitoneal injections of 1x105
.lymphoma cells and given 50 mg Kg–1 day–1 of methanolic extract of