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Sabrina deSouza was born in Dandenong, Victoria, Australia, grew up in Darwin, Northern Territory, and moved to Canberra, in the Australian Capital Territory before relocating to Queensland. She holds a Bachelor of Science degree, as well as an Honours Degree in Science. deSouza spent nine years as an Australian Customs Officer, the last seven of which were spent as an intelligence analyst. After deciding to leave government and join private industry, she went to work as a consultant and the principal trainer with Visual Analysis in Canberra before leaving to start her own consultancy business, Q3i2 in Queensland.